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WHAT IS  

JOINT SUPPORT?

Formulated with 100% herbal ingredients, HerbCiti Joint Support reduces joint imflammations, protect joint tissues and cartilaginous tissue, and relieves joint pain.

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BENEFITS

  • Significantly improves damaged cartilage, bones, tendons, and ligaments in fingers, wrists, and elbows due to rheumarthritis.

  • Enhances blood circulation, regenerates damaged tissues, reduces inflammations, and relieves joint pain. 

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JOINT SUPPORT IS RECOMMENDED FOR:

  • Patients with reuhmatoid arthrisis and gout

  • Those with lumbar spinal stenosis, shoulder pain, knee pain, and joint pain 

  • Those who are suffering from overworking and after effects from car accident

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WHAT IS A JOINT?

  • Joint is a structure in the human or animal body at which two parts of the skeleton are fitted together.

  • There are periosteum, ligament, joint cavity, articular cartilage, fibrous capsule and synovial membrane in a joint.

  • The main cause of arthritis is the accumulation of body waste and uric acid due to renal failure.

WHAT IS ARTHRITIS?

  • Arthritis is an inflammation of the joints, a very common disease among aging men and women. Generally, our joints slowly degenerate starting in age 40. Studies show that about 50% of men and women in their 60s have degenerative arthritis.

  • Osteoarthritis is the most common joint disease among aging men and women.

TYPES OF ARTHRITIS

RHEUMATOID ARTHRITIS IS A CHRONIC INFLAMMATION ON SYNOVIAL MEMBRANE.

  • Rheumatoid arthritis is characterized by "imflammation" of the synovium. Many people in their 30s and 40s have Rheumatoid arthritis. The ratio of female and male is about 3 to 1. Rheumatoid arthritis is caused by poisoning and inflammatory cells such as uric acid damaging our joint membrane.

  • Symptoms include stiffness, swelling, pain, and redness of joints.

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Gout is a form of arthritis caused by uric acid that is common in middle-aged men.

TYPES OF ARTHRITIS

Gout is a common inflammatory arthritis that can affect anyone. It's characterized by sudden, severe attacks of pain, swelling, redness and tenderness in the joints.

The higher the age, the higher the level of uric acid in the blood due to renal insufficiency.

Gout can occur at any age, but mostly occurs in men in their 40s and 50s.

More than 10 herbal medicines for joint protection and hematologic improvement, musculoskeletal enhancement, tissue regeneration, inflammation and pain relief, edema reduction, cartilage protection, anti-inflammatory and antioxidant effects.

JOINT SUPPORT

ANTI-INFLAMMATORY TEST

  • Raw264.7 cell strain -> LPS+RPMI or JS treatment -> measured TNFα concentration after 18 hours of culturing.

  • D10.G4.1 cell strain -> RPMI or JS treatment -> Measured IL-4 concentration after 48 hours of culturing.

  • Joint Support reduces the inflammatory cytokine TNFα in macrophage line called Raw264.7 concentration-dependently and increases the anti-inflammatory cytokine IL-4 in the Th2 cell line D10.G4.1, concentration-dependently.

ANTI-INFLAMMATORY TEST

  • Isolation of PBMC from Patients with Rheumatoid Arthritis -> PHA+RPMI, JS or MTX treatment -> Measured cytokine concentration after 48 hours of culturing.

  • Joint Support reduces the inflammatory cytokine IL-1β in PBMCs of patients with rheumatoid arthritis, while increasing the anti-inflammatory cytokine IL-4.

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IFN-γ in JS, MIP-1α regulatory effect/ RA patient PBMC

  • Isolation of PBMC from patients with Rheumatoid Arthritis -> PHA+RPMI, JS or MTX treatment -> Measured concentration of cytokine and chemokine after 48 hours of culturing.

  • Joint Support reduces inflammatory cytokines IFN-γ and chemokine MIP-1α in PBMCs of patients with rheumatoid arthritis.

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RANTES in JS, 

IL-8 regulatory effect/

RA patient PBMC

  • Isolation of PBMC from Patients with Rheumatoid Arthritis -> PHA+RPMI, JS or MTX treatment -> Measured concentration of chemokine after 48 hours of culturing.

  • Joint Support reduced the concentration level of chemokine Rantes and IL-8(arthritis-related) to the similar level of reduction by MTX.

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ICAM-1, sVCAM-1 in JS, regulatory effect/

RA patient PBMC

  • Isolation of PBMC from Patients with Rheumatoid Arthritis ->  PHA+RPMI, JS or MTX treatment -> Measured concentration of adhesion molecule after 48 hours of culturing.

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RANTES in JS,

MIP-1α regulatory effect/ RA patient SF

  • Isolation of synovial fibroblasts (SF) from patients with Rheumatoid Arthritis -> à TNFα+RPMI, JS or MTX treatment -> Measured concentration of chemokine after 48 hours of culturing.

  • Joint support significantly reduces the level of arthritis-related chemokine RANTES and MIP-1α in synovial fibroblasts isolated from the joint tissues of rheumatoid arthritis patients.

proMMP-1 in JS, MMP-3 regulatory effect/ RA patient SF

  • Isolation of synovial fibroblasts (SF) from patients with Rheumatoid Arthritis -> à TNFα+RPMI, JS or MTX treatment -> Measured concentration of proMMP-1, MMP-3 after 48 hours of culturing.

  • Joint support significantly reduces the concentrations of chondroitinases proMMP-1 and MMP-3 in synovial fibroblasts isolated from joint tissues of patients with rheumatoid arthritis.

Effectiveness of JS in rabbit OA model

  • Induced osteoarthritis with collagenase in a rabbit model -> Injected DW, JS or DCF -> Radiation measurement after 4th week and 8th week and began biological experiment.

  • Joint Support has shown to improve joint stiffness and prevent joint stenosis in the knee osteoarthritis rabbit model.

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Improvement of joint stenosis

  • Joint support has shown to prevent joint stenosis to a degree similar to DCF in rabbit osteoarthritis rabbit model.

Cartilage protection

  • Induced osteoarthritis with collagenase in a rabbit model -> Injected DW, JS or DCF -> Separated synovial fluids and tissues after 8th week -> Measured MMPs and TIMP.

  • Joint support has shown to reduce MMP-1 and MMP-3 manifestation in synovial tissues and increase the concentration of TIMP-2 in synovial fluids isolated from rabbit osteoarthritis.

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Cartilage protection

  • Induced osteoarthritis with collagenase in a rabbit model -> Injected DW, JS or DCF -> Separated synovial fluids and tissues after 8th week -> Measured MMPs and TIMP.

  • Joint Support reduces GAG levels by suppressing cartilage degradation in synovial fluid isolated from rabbit knee osteoarthritis.

Cartilage protection

  • Induced osteoarthritis with collagenase in a rabbit model -> Injected DW, JS or DCF -> Collected joint tissue after 8th week -> Safranine-O staining 

  • Joint Support reduces damage to cartilage proteoglycans in the knee osteoarthritis rabbit model.

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Cartilage protection

  • Induced osteoarthritis with collagenase in a rabbit model -> Injected DW, VM811 or DCF -> collected blood sample after 4th week -> Measured LTB4 and NO level.

  • Joint support has shown to reduce the level of LTB4 and NO(inflammatory mediators) similar to DCF, in both synovial fluid and blood collected from the knee osteoarthritis rabbit model.

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Anti-inflammatory Effects

  • Induced osteoarthritis with collagenase in a rabbit model -> Injected DW, JS or DCF -> Collected synovial fluids and blood sample after 4th week -> Measured PGE2 level.

  • Joint support reduces level of PGE2(inflammatory mediator) to the level similar to DCF in both the synovial fluid and blood sample collected from the knee osteoarthritis rabbit model.

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  • Joint support has shown similar effectiveness to the aspirin group by improving pain tolerance by more than 2 times (compared to the DW control group) in the pain model.

Pain Relief

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Safety Test

Rodent Oral Single Dose Toxicity Test (GLP) 1

Rodent 90 Day Oral Dose Toxicity Test (GLP) 2

Test Substance              JS (0g, 2g/5ml/kg)

& Dosage 

Animals Tested               5-week-old SD rats and 5 males and

                                                5 females per group

Number of Injection      1 time(s) at start

Test Substance              JS (0g, 2g/5ml/kg)

& Dosage 

Animals Tested               5-week-old SD rats and 5 males and

                                                5 females per group

Number of Injection      1 time(s) per day

Test Period                        14 days

Items Observed               mortality, body/organ weight, clinical signs,                                                  hematology profile, biochemical marker

Test Result                         No toxicity (estimated lethal dosage> 2g/kg)

Test Period                        90 days

Test Result                         No toxicity 

Items Observed               mortality, body/organ weight, clinical signs,                                                  hematology profile, biochemical marker

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Address:

19925 Stevens Creek Blvd. Suite 100

Cupertino, CA 95050

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Website:

www.herbcitibiz.com

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Email:

info@herbciti.com